Caspase-8 Colorimetric Assay Kit
This Caspase-8 Colorimetric Assay Kit is designed to detect caspase-8 cleaving activity, Caspase-8 (FLICE/MACH-1) is a 55 kDa cytosolic protein with homology to the CD95/Fas-associated signal transduction molecule, FADD, in addition to its homology with other caspases. The kit contains a caspase-8 fluorogenic substrate (Ac-IETD-pNA) and assay buffers. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate IETD-pNA. The pNA light emission can be quantified using a
spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in FLICE activity.
IETD-pNA Substrate: 500 μL
1 M dithiothreitol (DTT): 400 μL
Lysis Buffer: 100 mL
2X Reaction Buffer: 10 ml
Store at ≤ -20 °C for up to 6 months after initial use. Avoid repeated freeze-thaw cycles.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
There are no reviews yet.