Caspase-9 Colorimetric Assay Kit
The Caspase-9 Colorimetric Assay Kit is designed to provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase-9 activity.
LEHD-pNA (4 mM) Substrate: 500 μL
1 M dithiothreitol (DTT): 400 μL
Lysis Buffer: 100 mL
2X Reaction Buffer: 10 ml
Store at ≤ -20 °C for up to 6 months after initial use. Avoid repeated freeze-thaw cycles.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
There are no reviews yet.