For the best possible results, Celltechgen strongly recommends using our optimized application-specific protocols for each product. These protocols are the result of extensive in-house validation performed at Celltechgen and ensure accurate and reproducible results.

Western Blotting & Immunoprecipitation

Western Immunoblotting Protocol (Western)

Western Immunoblotting (Fluorescent) Protocol (Fluorescent Western)

Western Blot Reprobing Protocol (Western / Reprobe)

Immunoprecipitation Protocol / (For Denatured Proteins) (IP / Denatured Protein)

Immunoprecipitation Protocol / (For Native Protein) (IP / Native Protein)

Immunoprecipitation Protocol Utilizing Magnetic Separation / (For Analysis By Western Immunoblotting) (IP Magnetic)

Immobilized Immunoprecipitation Protocol / (For Analysis By Western Immunoblotting) (Immobilized IP)

Western Blotting Troubleshooting

Western Immunoblotting Troubleshooting Guide

Changes to Recommended Western Blotting Protocols


Fluorescent Multiplex Immunohistochemistry Protocol (mIHC/Paraffin)

Immunohistochemistry Troubleshooting

Immunohistochemistry Troubleshooting Guide


Immunofluorescence Protocol (IF Standard)

Immunofluorescence Protocol with Methanol Fixation

Immunofluorescence Protocol with Methanol Permeabilization (IF Methanol-perm)

Flow Cytometry

Flow Cytometry Protocol (Flow)

Flow Cytometry Troubleshooting

Flow Cytometry Troubleshooting Guide

Chromatin Immunoprecipitation

Chromatin Immunoprecipitation Troubleshooting

Chromatin Immunoprecipitation Troubleshooting Guide

Sandwich Immunoassays (ELISA)

ELISA-Peptide Assay Protocol (ELISA-P)

HRP Chemiluminescence ELISA-P (HRP Chemiluminescent ELISA-P)