Annexin V- iFluor™ 488 Apoptosis Detection Kit is used to quantitatively determine the percentage of cells within a population that are actively undergoing apoptosis. It relies on the property of cells to lose membrane asymmetry in the early phases of apoptosis. In apoptotic cells, the membrane phospholipid phosphatidylserine (PS) is translocated from the inner leaflet of the plasma membrane to the outer leaflet, thereby exposing PS to the external environment. Annexin V is a calcium-dependent phospholipid-binding protein that has a high affinity for PS, and is useful for identifying apoptotic cells with exposed PS. Propidium Iodide (PI) is a standard flow cytometric viability probe and is used to distinguish viable from nonviable cells. Viable cells with intact membranes exclude PI, whereas the membranes of dead and damaged cells are permeable to PI. Cells that stain positive for Annexin V- iFluor™ 488 and negative for PI are undergoing apoptosis. Cells that stain positive for both Annexin V- iFluor™ 488 and PI are either in the end stage of apoptosis, are undergoing necrosis, or are already dead. Cells that stain negative for both Annexin V- iFluor™ 488 and PI are alive and not undergoing measurable apoptosis.
Annexin V- iFluor™ 488: 0.5 ml
Propidium Iodide (PI): 2 ml
10X Annexin V Binding Buffer: 20 ml
Flow Cytometry, Fluorescence Microscopy
Store between 2°C-8°C. Do not freeze.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.