Caspase-3 Colorimetric Assay Kit
This Caspase-3 Colorimetric Assay Kit is designed to detect caspase-3(an early marker of cells undergoing apoptosis) or DEVD-cleaving activity, and determine if certain apoptosis pathways involve caspase-3 activation. The kit contains a caspase-3 fluorogenic substrate (Ac-DEVD-pNA) and assay buffers. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from
the labeled substrate DEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405-nm. Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in Caspase 3 activity.
Dilution Buffer (10X): 30 ml
Cell Lysis Buffer (1X): 30 ml
Reaction Buffer (2X): 10 ml
N-acetyl-Asp-Glu-Val-Asp-pNA: 0.5 ml
1 M DTT: 0.5 ml
Store at ≤ -20 °C for up to 6 months after initial use. Avoid repeated freeze-thaw cycles.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.