The Celltechgen™ Pyruvate dehydrogenase Assay kit （CTG-AK0032） provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc.In the pyruvate assay protocol, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured.
Pyruvate assay protocol summary:
– add samples and standards to wells
– add reaction mix and incubate for 30 min at room temp
– analyze with microplate reader
|sample||Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts|
|sensitivity||> 1 µM|
|detection concentrarion||1 µM – 200 µM|
Store at -20°C. Please refer to protocols.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.