Mitochondrial membrane potential (∆ψM) is one of the key parameter of mitochondrial function and serves as an indicator of cell health. JC‐1 Mitochondrial Membrane Potential Assay comprise of lipophilic cationic fluorescent dye; 5,5,6,6’‐tetrachloro‐1,1’,3,3’‐tetraethylbenzimi‐dazoylcarbocyanine iodide (JC‐1). In healthy cells, JC‐1 enters the energized mitochondria and form aggregates which change the fluorescent property of JC‐1 dye. JC‐1 monomers exhibit green fluorescence where as the JC‐1 dye aggregates also called J‐aggregates show intense red fluorescence. Unhealthy or apoptotic cells have low ∆ψM.JC‐1 does not form aggregates in mitochondria’s with low ∆ψM and remain in monomeric form and exhibit green fluorescence. Thus higher the ratio of red to green fluorescence, the higher is the polarization of mitochondrial membrane.
JC-1(5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimidazolylcarbocyanine iodide): Molecular formula: C25H27Cl4IN4; Molecular weight: 652.23
Mitochondrial membrane potential is important for many mitochondrial processes and is linked to cell health. The ∆ψM regulates ATP synthesis, ROS production, Calcium sequestration into mitochondria, import of mitochondrial proteins and mitochondrial membrane dynamics. On contrary, ∆ψM itself is controlled by ATP utilization, mitochondrial proton conductance, calcium levels in mitochondria and capacity of respiratory chains. Hence, mitochondrial and cell health are interrelated and mitochondrial membrane potential is one of the feature too look for when studying mechanisms related to cell health and when testing drugs.
100 Tests ; 200 Tests ; 500 Tests
5 vials of JC-1 (30 µg/vial of powdered dye)
1 vial of DMSO (1.5 mL)
1 vial of CCCP (125 µL)
1 bottle of 10 X phosphate-buffered saline (25 mL).
The kit is supplied at ambient temperature. Reconstitute the JC‐1 with DMSO (Sterile Filtered) provided in kit and aliquot in preferable one time use aliquots. Store the aliquots of JC‐1 at ‐20°C in dark. When stored as instructed, the kit is stable for 1 year.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.