Cat. No: P02-P1102Pr Size: 100 ug
Protein G, PerCP conjugated
Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.
Peridinin, a light-harvesting carotenoid, is associated with chlorophyll, which binds the protein subunits to form the peridinin-chlorophyll-protein light-harvesting complex (PerCP) in dinoflagellates, best studied in Amphidinium carterae.
Protein G, PerCP conjugated is a protein G labeled by PerCP.
Formulation: 100 µg of Protein G conjugated with PerCP in PBS.
Excitation Laser: Blue Laser (482 nm)
Application: In 0.1 ml PBS
Immunofluorescence (IF) 1: 50-1:200
Flow cytometry 1: 100-1:500.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procdures.