Cat. No: P02-P1102A Size: 100 ug
Protein G, cl-APC conjugated
Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.
Allophycocyanin (APC), purified from Spirulina, is made up of alpha and beta subunits and is present as a trimer (αβ)3, which is unstable and susceptible to dissociation at low concentrations. The monomer, αβ, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is also shifted to 620 nm. The chemically cross-linked APC trimer (cl-APC) is much more stable than the native APC trimer, but still retains the same spectroscopic properties as the native APC trimer.
Protein G, cl-APC conjugated is a protein G labeled by cl-APC
Formulation: 100 µg of Protein G conjugated with cl-APC in PBS.
Excitation Laser: Red Laser (651 nm)
Application: In 0.1 ml PBS
Immunofluorescence (IF) 1: 50-1:200
Flow cytometry 1: 100-1:500.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procdures.