Streptavidin, PerCP conjugated
Catalog Number: AX-AP0010
Size: 0.1 mg (0.2mg/ml)
Absorption Maximum: 478 nm
Emission Maximum: 675 nm
Streptavidin-PerCP is produced as the PerCP derivative of streptavidin and is provided in a ready-to-use liquid format containing up to 0.5% bovine serum albumin as stabilizer.
Streptavidin-PerCP can be used to label biotinylated proteins/antibodies and biotin derivatized surfaces ias secondary detection reagents in histochemical applications, flow cytometry, blot analysis, and immunoassays. These reagents can also be employed to localize biocytin, biocytin-X, biotin ethylenediamine and so on. The following are commonly used methods for employing streptavidin as a secondary detection reagent.
A biotinylated primary molecules such as an antibody is bound to tissues, cells, or other surfaces. Excess protein is removed by washing, and detection is mediated by adding Streptavidin-PerCP. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the PerCP conjugation. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Store reagent at 2° – 8° C. DO NOT FREEZE. Reagent is stable for at least 6 months after purchase. For research or further manufacturing use only.
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