Propidium iodide (PI) is a popular red-fluorescent nuclear and chromosome counterstain. Since propidium iodide is not permeant to live cells, it is also commonly used to detect dead cells in a population. PI binds to DNA by intercalating between the bases with little or no sequence preference. In aqueous solution, the dye has excitation/emission maxima of 493/636 nm. Once the dye is bound, its fluorescence is enhanced 20- to 30-fold, the fluorescence excitation maximum is shifted ~30–40 nm to the red and the fluorescence emission maximum is shifted ~15 nm to the blue, resulting in an excitation maximum at 535 nm and fluorescence emission maximum at 617 nm.PI is widely used in fluorescence microscopy, confocal laser scanning microscopy, flow cytometry, and fluorometry.
Propidium iodide solid , 100 mg or 10 ml of Propidium iodide solution, 1 mg/mL in water
Flow Cytometry, Fluorescence Microscopy
Store Propidium iodide solid at Room temperature, protect from light. When stored as directed, product is stable at least 1 year. Store Propidium iodide solution (P3566) 1 mg/mL in water 2–6°C, protect from light. When stored as directed, solution is stable at least 6 months.
Celltechgen provides high-quality reagents and materials for research use only. PI is a potential mutagen and should be handled with care. The dye must be disposed of safely and in accordance with applicable local regulations. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
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