The LDH Cytoxicity Detection is a colorimetric measure of cell cytotoxicity/cytolysis based on the measurement of LDH activity in the cell culture supernatant. LDH activity is determined via the Diaphorase enzymatic reaction in which NAD+ is reduced to NADH/H+ by the LDH-catalyzed conversion of lactate to pyruvate, the H/H+ from the resulting NADH/H+ is transferred to the yellow tetrazolium salt INT, which becomes reduced to a red formazan product. An increase in the number of dead or plasma membrane-damaged cells results in an increase in the total LDH enzymatic activity in the cell culture supernatant. This increase in LDH activity directly correlates with the amount of formazan product formed. In this assay, the value of spectral absorption of the resulting formazan at ~490nm is directly proportional to the number of damaged cells.
Detection and quantification of cell-mediated cytotoxicity induced by cytotoxic T-lymphocytes (CTL), natural killer (NK) cells, lymphokine activated killer (LAK) cells or monocytes; Determination of factors that lead to cytolysis; Measurement of antibody-dependent cellular cytotoxicity (ADCC) and complement mediated cytolysis; Determination of the cytotoxic potential of compounds in environmental and medical research and in the food, cosmetic, and pharmaceutical manufacturing; Determination of cell death in bioreactors.
200tests ; 400tests ; 800tests
- Substrate Mix, 5X lyophilizate
- 30 ml Assay Buffer
- 10 ml 1X Lysis Buffer, 2.5mL
- Stop Solution, 20 mL
- LDH Positive Control, 6µL
Store the kit at 15 to 25°C where it will be stable until the expiration date printed on the label.
The lyophilizate (bottle 1) is stable at +2 to +8°C. The reconstituted catalyst solution is stable for four weeks when stored +2 to +8°C. Once thawed, the dye solution (bottle 2) is stable for several weeks when stored at +2 to +8°C.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.