The Celltechgen™ RIPA Lysis Buffer is one of the most reliable buffers used to lyse cultured mammalian cells from both plated cells and cells pelleted from suspension cultures. This buffer enables protein extraction from cytoplasmic, membrane and nuclear proteins and is compatible with many applications, including reporter assays, protein assays, immunoassays and protein purification.
• RIPA Buffer does not contain protease or phosphatase inhibitors. If desired, add protease inhibitors, such as Thermo Scientific Halt Protease Inhibitor Cocktail and Halt™ Phosphatase Inhibitor Cocktail to the reagent to prevent proteolysis and maintain phosphorylation status of proteins. Add protease and phosphatase inhibitors immediately before use.
• Use 1mL of cold RIPA Buffer for every 5 × 106 of HeLa or A431 cells (~20µL of packed cells, which is equivalent to ~40mg of cells). To obtain concentrated protein extracts, directly lyse cells on plate and use less buffer.
• Some protein kinases and other enzymes may be sensitive to the components of the RIPA Buffer, resulting in their decreased activity. In such cases, prepare a RIPA buffer that does not contain sodium deoxycholate and SDS.
• RIPA Buffer is compatible with the The Celltechgen™ RIPA Lysis Buffer BCA Protein Assay Kit .
50ml ; 100ml ; 250ml
Upon receipt store at 4°C. Product shipped at ambient temperature.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.