G 418 is an aminoglycoside antibiotic similar in structure to gentamycin. It exhibits toxicity towards both eukaryotic and prokaryotic cells. The optimal concentration for selection and maintenance must be determined for each cell line. For bacteria and algae, concentrations of 5 µg/ml or less are recommended. Animal cells may require up to 300-500 µg/ml. Typically, resistance is conferred by one of two dominant genes of bacterial origin, which can be expressed in eukaryotic cells. Cells that are multiplying will be affected sooner than those that are not. Cells in log phase may require three to seven days for selection. In general, concentrations of approximately 400 µg/ml for selection and 200 µg/ml for maintenance are required for mammalian cells.
Synonym: Antibiotic G418
CAS Number 108321-42-2
Empirical Formula (Hill Notation) C20H40N4O10 · 2H2SO4
Molecular Weight 692.71
MDL number MFCD00058314
PubChem Substance ID 24890581
Specific Rotation: +104.4° (c = 0.3% in H2O at 26 °C)
The G 418 powder is stable for three years as supplied when stored at 2-8 °C. The product is stable for three years when stored at 2-8°C.
1, 5g in glass bottle
G418 powder is soluble in water at 50 mg/mL.
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