Aprotin,also known as pancreatic trypsin inhibitor and trypsin-kallikrein inhitor,is found in a number of organs,e.g.
- Liver and
And is also be detectable in free form in calf serum.An intracellular from has also been identified.Its unique structure is responsible for the molecule’s high stability and remarkable resistance to elevated temperature,acids and proteases
Aprotinin is a monomeric polypeptide (58 amino acids)with the following sequence:
RPDFCLEPPY TGPCKARIIR YFYNAKAGLC
QTFVYGGCRA KRNNFKSAEN CMRTCGGA
The two-dimensional conformation is maintained by three disulfidebridges Cys5-Cys55, Cys14-Cys38 und Cys30-Cys51.In the trypsin-aprotinin complex the following amino acids appear to be responsible for the strong binding of the inhibitor to the enzyme: lysine at position 15 and alanine at position 16 in the active center of the enzyme and two arginine moieties at positions 17 and 39. (7,8,9).
|Molecular weight||Mr = 6512|
|Absorbance||A280 nm, 1 cm (1 mg/ml) = 0.84|
|Solubility||Freely soluble in water (10mg/ml) and aqueous buffer solution(e.g.0.1 M Tris buffer, pH 8.0).|
Store at 2 ℃-8℃
25mg ; 100mg
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