Cat. No: P02-P1102A Size: 100 ug
Protein G, cl-APC conjugated
Introduction:
Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.
Allophycocyanin (APC), purified from Spirulina, is made up of alpha and beta subunits and is present as a trimer (αβ)3, which is unstable and susceptible to dissociation at low concentrations. The monomer, αβ, has a lower fluorescence quantum yield compared to the trimer and the maximal absorption is also shifted to 620 nm. The chemically cross-linked APC trimer (cl-APC) is much more stable than the native APC trimer, but still retains the same spectroscopic properties as the native APC trimer.
Protein G, cl-APC conjugated is a protein G labeled by cl-APC
Formulation: 100 µg of Protein G conjugated with cl-APC in PBS.
Excitation Laser: Red Laser (651 nm)
Application: In 0.1 ml PBS
Immunofluorescence (IF) 1: 50-1:200
Flow cytometry 1: 100-1:500.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Specificity:
Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.
Usage:
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procdures.
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