Protein A/G, R-PE Conjugated
Catalog Number: P02-P0116
Size: 100 µg
Description: Approximately 50.5 kDa. Recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to Protein A and G domains that are included in the recombinant sequence. Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. Protein G portion is from Streptococcus segments C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.
Phycoethyrin (R-PE): R-PE is a 240 kDa protein from the light-harvesting complex, which is composed of a hexameric structure of alpha and beta chains andphycobilins. R-PE purified from Poryhyratenera.
Protein A/G, R-PE conjugate is a protein labled by Phycoethyrin.
Formulation: 100 µg of Protein A conjugated with R-PE in PBS.
Excitation Laer: Blue Laser (488 nm)/Green Laser (532 nm)/Yellow-Green Laser (566 nm)
Specificity: The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.
Applications: Protein A/G is an excellent tool for purification and detection of mouse monclonal antibodies from IgG subclasses without interference from these other serum proteins. Individual subclasses of mouse monoclonals are most likely to have stronger affinity to this chimeric protein than to either Protein A or Protein G. Binding is less pH-dependent than either Protein A or Protein G alone, occurring well at pH 5-8.
Immunofluorescence (IF) 1: 50-1:200
Flow cytometry 1: 100-1:500.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Storage: 1 year at 2-8°C. Don’t freeze. Product shipped with an ice pack.
Usage: This material is for research, laboratory or further evaluation purposes.
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