The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Hygromycin B acts against bacteria, fungi and higher eukaryotic cells.
Synonym: Hygromycin B from Streptomyces hygroscopicus
CAS Number 31282-04-9
Empirical Formula (Hill Notation) C20H37N3O13
Molecular Weight 527.52
MDL number MFCD06795479
PubChem Substance ID 329749191
50 – 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used. LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
For life science research only. Not for use in diagnostic procedures.
Purity: 97.3% (HPLC)
20 ml Solution, 50 mg/ml in PBS (phosphate-buffered saline), filtered through 0.2 μm pore size membrane
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain the phenotype of eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph). Recommended concentration for the selection of resistant cells is 50 to 1000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be determined experimentally, and may vary with the type of cell used.