DESCRIPTION
Thermo Scientific DreamTaq Green PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq Green buffer, MgCl2, and dNTPs. The master mix retains all features of DreamTaq DNA Polymerase. It is capable of robust amplification of up to 6 kb from genomic DNA and up to 20 kb from viral DNA. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. Extensive optimization of reaction conditions is not required.
Polymerase: | Taq DNA Polymerase |
Hot Start: | No |
Fidelity (vs. Taq): | 1 X |
GC-Rich PCR Performance: | Low |
Reaction Format: | SuperMix or Master Mix |
Product Overhang: | 3′-A |
Reaction Speed: | Standard |
Highlights
• Direct loading of PCR product on gel
• High yields and high sensitivity of PCR
• Amplification of long targets up to 6 kb from genomic DNA and up to 20 kb from viral DNA
• Robust amplification with minimal optimization
• Incorporates modified nucleotides, but does not incorporate dUTP
Contains:
• DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• Nuclease-free water
Applications
• Routine PCR amplification of DNA fragments up to 6 kb
• Genotyping
• High throughput PCR
STORAGE
Store at -20℃
SIZE
200 Reactions
1000 Reactions
USAGE
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
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