DESCRIPTION
The First Strand Cdna Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.The kit uses RevertAid Reverse Transcriptase (RT),which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant Thermo Scientific™ RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C. First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.
STORAGE
All components of the kit should be stored at -20°C. Keep control RNA at -70°C for longer storage.
SIZE
20 Tests ;100Tests
Usage
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
Reviews
There are no reviews yet.