The First Strand Cdna Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.The kit uses RevertAid Reverse Transcriptase (RT),which has lower RNase H activity compared to AMV reverse transcriptase. The enzyme maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb. The recombinant Thermo Scientific™ RiboLock™ RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C. First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR. It is also ideal for second strand cDNA synthesis or linear RNA amplification. Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays.
All components of the kit should be stored at -20°C. Keep control RNA at -70°C for longer storage.
20 Tests ;100Tests
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.