The Celltechgen™ Caspase-3 Fluorometric Assay Kit
$450.00
DESCRIPTION
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The CPP32/Caspase-3 Fluorometric Protease Assay Kit provides a simple and convenient means for assaying the DEVD-dependent caspase activity. The assay is based on detection of cleavage of substrate DEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). DEVD-AFC emits blue light (λmax = 400 nm); upon cleavage of the substrate by CPP32 or related caspases, free AFC emits a yellow-green fluorescence (lmax = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity.
| Detection Method | Fluorecence (400 nm excitation filter and 505 nm emission filter) |
| Species Reactivity | Mammalian |
| Applications | Applications |
| Features & Benefits | • Simple one-step procedure; takes 1-2 hours • Fast and convenient • Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-3/CPP32 activity |
| Kit Components | • Cell Lysis Buffer • 2X Reaction Buffer • DEVD-AFC (1 mM) • DTT (1 M) |
SIZE
20tests ; 50tests ; 100tests
STORAGE
Store at -20℃ ； Shipping with Gel Pack
USAGE
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
| Categories | |
|---|---|
| Applications | Apoptosis |
| Research area | |
| Size |
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