Recombinant Protein L

Protein L

Introduction:

Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Recombinant Protein L Protein, fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. DTT-reduced protein migrates as a 36 to 38 kDa polypeptide.

Size: 5 mg

Purity: >98% by SDS-PAGE

Endotoxin level:  <0.1 EU/mg of Protein L.

Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Storage: Avoid repeated freeze-thaw cycles, in lyophilized state for 1 year store at 4^oC-8^oC for 1 year in lyophilized state; store at 4^oC-8^oC for 1 month after reconstitution under sterile conditions or 3 months at -20^oC to -70^oC.

Specificity:
Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies.

Usage:
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

IgG Binging to Protein A, G and L:

Strong binding ＋＋, medium interaction ＋, weak or no interaction －.