Protein L
Introduction:
Protein L was isolated from the surface of bacterial species Peptostreptococcus magnus and was found to bind Ig(IgG,IgM,IgA,IgE and IgD) through L chain interaction. The recombinant protein contains four immunoglobulin (Ig) binding domains (Bdomains) of the native protein. Recombinant Protein L Protein, fused with the polyhistidine tag at N-terminus and a single cysteine at C-terminus. DTT-reduced protein migrates as a 36 to 38 kDa polypeptide.
Size: 5 mg
Purity: >98% by SDS-PAGE
Endotoxin level: <0.1 EU/mg of Protein L.
Formulation: Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
Storage: Avoid repeated freeze-thaw cycles, in lyophilized state for 1 year store at 4oC-8oC for 1 year in lyophilized state; store at 4oC-8oC for 1 month after reconstitution under sterile conditions or 3 months at -20oC to -70oC.
Specificity:
Despite this wide-ranging binding capability with respect to Ig classes, Protein L is not a universal immunoglobilin-binding protein. Binding of Protein L to immunoglobulins is restricted to those containing kappa light chains (i.e., k chain of the VL domain). Besides antibody, protein L is also suitable for binding of a wide range of antibody fragments such as Fabs, single-chain variable fragments (scFv), and domain antibodies.
Usage:
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
IgG Binging to Protein A, G and L:
Species | Protein A | Protein G | Protein L | |
Human | IgG | +++ | +++ | +++ |
IgG1 | ++++ | ++++ | ++++ | |
IgG2 | ++++ | ++++ | ++++ | |
IgG3 | – | +++ | +++ | |
IgG4 | ++++ | ++++ | ++++ | |
IgA | + | – | +++ | |
IgA1 | + | – | +++ | |
IgA2 | + | – | +++ | |
IgD | – | – | +++ | |
IgE | ++ | – | +++ | |
IgM | + | – | +++ | |
Rabbit | IgG | +++ | +++ | + |
Cow | IgG | + | +++ | – |
IgG1 | + | +++ | – | |
IgG2 | +++ | +++ | – | |
Cat | IgG | +++ | + | ? |
Horse | IgG | ++ | ++++ | ? |
Goat | IgG | + | ++ | – |
IgG1 | + | +++ | – | |
IgG2 | +++ | +++ | – | |
Guinea-pig | IgG1 | ++ | + | ? |
IgG2 | ++ | + | ? | |
Sheep | IgG | + | ++ | – |
IgG1 | + | ++ | – | |
IgG2 | +++ | +++ | – | |
Dog | ++ | + | ? | |
Pig | +++ | ++ | +++ | |
Rat | + | ++ | +++ | |
IgG1 | – | + | +++ | |
IgG2a | – | ++++ | +++ | |
IgG2b | – | ++ | +++ | |
IgG2c | ++ | ++ | +++ | |
IgG3 | + | ++ | ? | |
Mouse | IgG | ++ | ++ | +++ |
IgG1 | + | ++++ | +++ | |
IgG2a | ++++ | ++++ | +++ | |
IgG2b | +++ | +++ | +++ | |
IgG3 | ++ | +++ | +++ | |
IgM | – | – | +++ | |
Chicken | IgY | – | – | – |
Monkey | IgG | ++++ | ++++ | ? |
Hamster | + | ++ | +++ | |
Koala | – | + | ? | |
Llama | – | + | ? |
Strong binding ++, medium interaction +, weak or no interaction ï¼.
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