Recombinant Protein G
Catalog Number: AX-AP1102
Size: 5 mg
Source: Escherichia coli
Description: Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.
Purity: >95% by SDS-PAGE analyses.
Specificity: Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.
Applications: The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2.
Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation: Lyophilized with no additive.
Endotoxin: Less than 0.1EU/μg of Protein G as determined by LAL method.
Reconstitution: Dissolve in distilled water or saline.
Storage: 2 years at -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
Usage: This material is for research, laboratory or further evaluation purposes.
IgG Binging to Protein A, G and L:
Species | Protein A | Protein G | Protein L | |
Human | IgG | +++ | +++ | +++ |
IgG1 | ++++ | ++++ | ++++ | |
IgG2 | ++++ | ++++ | ++++ | |
IgG3 | – | +++ | +++ | |
IgG4 | ++++ | ++++ | ++++ | |
IgA | + | – | +++ | |
IgA1 | + | – | +++ | |
IgA2 | + | – | +++ | |
IgD | – | – | +++ | |
IgE | ++ | – | +++ | |
IgM | + | – | +++ | |
Rabbit | IgG | +++ | +++ | + |
Cow | IgG | + | +++ | – |
IgG1 | + | +++ | – | |
IgG2 | +++ | +++ | – | |
Cat | IgG | +++ | + | ? |
Horse | IgG | ++ | ++++ | ? |
Goat | IgG | + | ++ | – |
IgG1 | + | +++ | – | |
IgG2 | +++ | +++ | – | |
Guinea-pig | IgG1 | ++ | + | ? |
IgG2 | ++ | + | ? | |
Sheep | IgG | + | ++ | – |
IgG1 | + | ++ | – | |
IgG2 | +++ | +++ | – | |
Dog | ++ | + | ? | |
Pig | +++ | ++ | +++ | |
Rat | + | ++ | +++ | |
IgG1 | – | + | +++ | |
IgG2a | – | ++++ | +++ | |
IgG2b | – | ++ | +++ | |
IgG2c | ++ | ++ | +++ | |
IgG3 | + | ++ | ? | |
Mouse | IgG | ++ | ++ | +++ |
IgG1 | + | ++++ | +++ | |
IgG2a | ++++ | ++++ | +++ | |
IgG2b | +++ | +++ | +++ | |
IgG3 | ++ | +++ | +++ | |
IgM | – | – | +++ | |
Chicken | IgY | – | – | – |
Monkey | IgG | ++++ | ++++ | ? |
Hamster | + | ++ | +++ | |
Koala | – | + | ? | |
Llama | – | + | ? |
Strong binding ++, medium interaction +, weak or no interaction ï¼.
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