Recombinant Protein G

Recombinant Protein G

Catalog Number: AX-AP1102

Size: 5 mg

Source: Escherichia coli

Description: Recombinant streptococcal protein G lacking the albumin binding region thereby avoiding undesirable reactions with albumin, though the Fc binding domain is still present. The recombinant Protein G is produced in Escherichia coli using sequence from Streptococcus C1-C2-C3. The Protein G contains 196 amino acids (190-384) having a molecular mass of 21.6 kDa. The Protein-G migrates on SDS-PAGE around 30 kDa.

Purity: >95% by SDS-PAGE analyses.

Specificity: Protein G binds with greater affinity to most mammalian immunoglobulins than Protein A, including human IgG3 and rat IgG2a, and does not bind to human IgM, IgD and IgA.

Applications: The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2.

Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation: Lyophilized with no additive.

Endotoxin: Less than 0.1EU/μg of Protein G as determined by LAL method.

Reconstitution: Dissolve in distilled water or saline.

Storage: 2 years at -20°C. After reconstitution, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Usage: This material is for research, laboratory or further evaluation purposes.

IgG Binging to Protein A, G and L:

Strong binding ＋＋, medium interaction ＋, weak or no interaction －.