Recombinant Mouse Complement Component C5a Protein

Size: 10 ug

Source

E. coli-derived mouse Complement Component C5a protein: Asn679-Arg755, with an N-terminal Met

Accession number

P06684

N-­terminal Sequence Analysis

Met

Molecular Weight

9.0 kDa, reducing conditions (SDS-PAGE)

Activity

Measured by its ability to induce N-acetyl-beta -D-glucosaminidase release from differentiated U937 human histiocytic lymphoma cells. The ED50 for this effect is 5-20 ng/mL.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Purity

95%, by SDS­PAGE.

Formulation

Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Sucrose. See Certificate of Analysis for details.

Shipping & Storage

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. Avoid repeated freeze-­thaw cycles. Store at ­70 °C for 6 months from date of receipt, store ­at 70 °C under sterile conditions after opening for 6 months.