Purified Recombinant Annexin V
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic cells and necrotic. Therefore, we recommend using our 7-AAD Viability Staining Solution or Propidium Iodide Solution. Early apoptotic cells will exclude 7-AAD and PI, while late stage apoptotic cells and necrotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.
Size: 100 ug
Recombinant Annexin V was expressed in bacteria and is ≥95% pure as determined by SDS/PAGE stained with Coomassie Blue.
Store product at -80°C prior to use or for long term storage of stock solutions. Avoid multiple freeze-thaws of product.