Cat. No: AX-AP1100Pr Size: 100 ug
Protein A, PerCP conjugated
Protein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E.Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series. It migrates with an apparent molecular mass of 45kDa in SDS-PAGE.
Peridinin, a light-harvesting carotenoid, is associated with chlorophyll, which binds the protein subunits to form the peridinin-chlorophyll-protein light-harvesting complex (PerCP) in dinoflagellates, best studied in Amphidinium carterae.
Protein A, PerCP conjugated is a protein A labeled by PerCP.
Formulation: 100 µg of Protein A conjugated with PerCP in PBS.
Excitation Laser: Blue Laser (482 nm)
Application: In 0.1 ml PBS
Immunofluorescence (IF) 1: 50-1:200
Flow cytometry 1: 100-1:500.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
The interaction between Protein A and IgG is not equivalent for all species. Even within a species, Protein A interacts with some subclasses of IgG and not others. For instance, human IgG1, IgG2 and IgG4 bind strongly, while IgG3 does not bind. There are also many instances in which monoclonal antibodies do not bind to Protein A, especially the majority of rat immunoglobulins and mouse IgG1.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procdures.