Cat. No: AX_PI_0011
Size: 1 ml
Protease Inhibitor Cocktail (100X)
Protease Inhibitor Cocktails are 100X solutions containing optimized concentrations of six broad-spectrum protease inhibitors stabilized in high-quality dimethylsulfoxide (DMSO). The cocktails effectively inhibit serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases that are typically present in cellular lysate samples. Regular and EDTA-free formulations provide for inhibition of metalloproteases and compatibility with 2D electrophoresis, respectively.
Formulation and concentration of the Protease Inhibitor Cocktail.
Inhibitor MW Protease Family Targeted Inhibitor Type Concentration
Component in 100X Cocktail
AEBSF•HCl 239.5 Serine proteases Irreversible 100mM
Aprotinin 6511.5 Serine proteases Reversible 80µM
Bestatin 308.38 Amino-peptidases Reversible 5mM
E-64 357.4 Cysteine proteases Irreversible 1.5mM
EDTA* 372.24 Metalloproteases Reversible 0.5M †
Leupeptin 475.6 Serine and cysteine proteases Reversible 2mM
Pepstatin A 685.9 Aspartic acid proteases Reversible 1mM
*EDTA is not present in the EDTA-Free Cocktail formulation.
Storage: Upon receipt store product at 4°C. Product is shipped at ambient temperature.