Phospho JNK1/2 (T183/Y185) Flex Set

Phospho JNK1/2 (T183/Y185) Flex Set
Size: 100 Tests
Assay Range: 3.9-1,000 Units/mL
Reactivity: Human

INTENDED USE

This Phospho JNK1/2 (T183/Y185) Flex Set is used to human, mouse, or rat c-Jun N-terminal kinase1/2 (JNK1/2), that has been threonine-phosphorylated at Thr-183 and tyrosine-phosphorylated at Tyr-185 in denatured cell lysate samples. The Reagent Diluent recommended may be suitable for most cell culture supernate, serum, and plasma samples.

This kit contains sufficient materials to run 100 tests, provided the following conditions are met:
• The reagents are prepared as described in this package insert.
• The assay is run as described in the ASSAY PROCEDURE.
• The recommended microplates, buffers, diluents, substrates, and solutions are used.

This package insert must be read in its entirety before using this product. For research use only. Not for use in diagnostic procedures.

DESCRIPTION
The Phospho JNK1/2 (T183/Y185) Flex Set is a bead-based immunoassay capable of measuring human, mouse, or rat c-Jun N-terminal kinase1/2 (JNK1/2), that has been threoninephosphorylated at Thr-183 and tyrosine-phosphorylated at Tyr-185 in denatured cell lysate samples. Human and mouse reactivity was determined by testing cell lysates with the Phospho JNK1/2 (T183/Y185) Flex Set.

ITEMS PROVIDED
1. Phospho JNK1/2 (T183/Y185) PE Detection Reagent
Storage Buffer: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
2. Phospho JNK1/2 (T183/Y185) Standard
Storage Buffer: Lyophilized in an aqueous buffered solution containing BSA and ProClin™ 150.
3. Phospho JNK1/2 (T183/Y185) Capture Bead B5
Storage Buffer: Aqueous buffered solution containing fetal bovine serum and ≤0.09% sodium azide.

PREPARATION AND STORAGE

This Flex Set contains one vial of each component listed above. All components of this flex set have been formulated to a 50x concentration to ensure product performance when multiplexed. Store at 4°C. Protect Capture Beads and the PE Detection Reagent from prolonged exposure to light. The Phospho JNK1/2 (T183/Y185) Standard provided in this Flex Set is lyophilized and the standard sphere should be transferred to a 1.5 mL microfuge tube for reconstitution. Reconstitute the standard with 0.1 mL Assay Diluent from the Cell Signaling Master Buffer Kit, warm to 37 °C and vortex prior to use. After reconstitution, the standard concentration is 50,000 Units/mL and is stable for 3 months when stored at 4 °C. When using reconstituted standard, warm to 37 °C and vortex to mix thoroughly before use.

APPLICATION NOTES

The Phospho JNK1/2 (T183/Y185) Flex Set must be used in conjunction with a Cell Signaling Master Buffer Kit and a flow cytometer. Detailed instructions on the use of this product can be found in the manual for the Cell Signaling Master Buffer Kit. When following the directions in the Master Buffer Kit, the top standard point for the Phospho JNK1/2 (T183/Y185) Flex Set will be 1,000 Units/mL.