Myeloperoxidase (MPO) Colorimetric Activity Assay Kit is a rapid, simple, sensitive, and reliable colorimetric assay suitable for use as a high throughput MPO activity assay. In the MPO assay protocol, myeloperoxidase produces HClO from H2O2 and Cl–. Myeloperoxidase (MPO) is a peroxidase enzyme (EC 220.127.116.11) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In Celltechgen’s MPO colorimetric Assay Kit, MPO catalyzes the formation of hypochlorous acid, which reacts with taurine to form taurine chloroamine. Taurine chloroamine reacts with the chromophore TNB, resulting in the formation of the colorless product DTNB. One unit of MPO activity is defined as the amount of enzyme that hydrolyzes the substrate and generates taurine chloramine to consume 1.0 µmole of TNB per minute at 25 oC. Myeloperoxidase peroxidation activity, please check Celltechgen’s Myeloperoxidase Peroxidation Activity Assay Kit (Cat.NO:CTG-AK0037)
MPO Colorimetric Activity Assay Kit has been used to determine the activity of myeloperoxidase in samples.
MPO Assay Buffer 25 ml
MPO Substrate Stock 50 µl
DTNB Probe 50 µl
Stop Mix 20 µl
MPO Positive Control 1 vial (MPO)
TCEP, 50 mM 50 µL
Store kit at -20°C protected from light. Allow Assay Buffer to warm to room temperature
before use. Briefly centrifuge small vials before opening.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.