MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme. MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents. It is expressed in normal human tissues and is overexpressed in many types of tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy. MGMT overexpression is associated with BCNU (carmustine) resistance in malignant gliomas.
KIT COMPONENTS (100 reactions)
- 50x DAB Chromogen 1 x 0.5ml
- Biotinylated goat anti-rabbit IgG(H+L) 1 x 15ml
- DAB substrate 1 x 15ml
- Hydrogen Peroxide Block 1 x 15ml
- Protein block 1 x 15ml
- Streptavidin Peroxidase 1 x 15ml
- Rabbit anti-human MGMT
Store at +4°C. Please refer to protocols.
The products is “”FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES”””