Caspase-9 Fluorometric Assay Kit
The Caspase-9 Fluorometric Assay Kit is designed to provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on detection of cleavage of substrate LEHD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEHD-AFC emits blue light (Emax = 400 nm); upon cleavage of the substrate by caspase-9 or related caspases, free AFC emits a yellow-green fluorescence (Emax =505 nm), which can be quantified using a fluorometer or fluorecence microtiter plate reader. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-9 activity.
LEHD-AFC Substrate: 500 μL
1 M dithiothreitol (DTT): 400 μL
Lysis Buffer: 100 mL
2X Reaction Buffer: 10 ml
Store at ≤ -20 °C for up to 6 months after initial use. Avoid repeated freeze-thaw cycles.
Celltechgen provides high-quality reagents and materials for research use only. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.