The Bradford method is a Dye-based assay and is based on the ability of Coomassie blue G-250 to bind protein causing the dye to shift from a red colour to a blue colour. In the acidic environment of the reagent, protein binds to the Coomassie dye. This results in a spectral shift from the reddish/brown form of the dye (absorbance maximum at 465 nm) to the blue form of the dye (absorbance maximum at 610 nm). The difference between the two forms of the dye is greatest at 595 nm, so that is the optimal wavelength to measure the blue colour from the Coomassie dye-protein complex. If desired, the blue colour can be measured at any wavelength between 575 nm and 615 nm.
The Bradford assay is the fastest and easiest to perform among the protein assays and uses about the same amount of protein as the Lowry assay. It is also compatible with most salts, solvents, buffers, thiols, reducing substances and metal chelating agents. However, If the protein sample to be assayed has detergents present in the buffer, it is suggested to use the BCA protein determination procedure. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assessing protein concentrations for gel electrophoresis.
Sufficient For: 630 tube assays or 3800 microplate assays
- Pierce Coomassie Assay Reagent, 950 mL
- Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL
This product is for R&D use only, not for drug, household, or other uses. Please consult the Material
Safety Data Sheet for information regarding hazards and safe handling practices.
The product is stored at 2–8 °C. It is stable at 2–8 °C in an unopened container for at least 1 year. Store unopened Albumin Standard Vials at room temperature.
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