Biotinylated Recombinant Protein A/G
Catalogue #: AX-AP1101B
Size: 0.5 mg
Description: Approximately 50.5 kDa. Recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to Protein A and G domains that are included in the recombinant sequence. Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. Protein G portion is from Streptococcus segments C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.
Specificity: Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG. The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.
Formulation: Lyophilized from a 0.2μm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C.
Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C.Avoid repeated freeze/thaw cycles.
Usage: For research, laboratory or further evaluation purposes.
IgG Binging to Protein A, G and L:
Species | Protein A | Protein G | Protein L | |
Human | IgG | +++ | +++ | +++ |
IgG1 | ++++ | ++++ | ++++ | |
IgG2 | ++++ | ++++ | ++++ | |
IgG3 | – | +++ | +++ | |
IgG4 | ++++ | ++++ | ++++ | |
IgA | + | – | +++ | |
IgA1 | + | – | +++ | |
IgA2 | + | – | +++ | |
IgD | – | – | +++ | |
IgE | ++ | – | +++ | |
IgM | + | – | +++ | |
Rabbit | IgG | +++ | +++ | + |
Cow | IgG | + | +++ | – |
IgG1 | + | +++ | – | |
IgG2 | +++ | +++ | – | |
Cat | IgG | +++ | + | ? |
Horse | IgG | ++ | ++++ | ? |
Goat | IgG | + | ++ | – |
IgG1 | + | +++ | – | |
IgG2 | +++ | +++ | – | |
Guinea-pig | IgG1 | ++ | + | ? |
IgG2 | ++ | + | ? | |
Sheep | IgG | + | ++ | – |
IgG1 | + | ++ | – | |
IgG2 | +++ | +++ | – | |
Dog | ++ | + | ? | |
Pig | +++ | ++ | +++ | |
Rat | + | ++ | +++ | |
IgG1 | – | + | +++ | |
IgG2a | – | ++++ | +++ | |
IgG2b | – | ++ | +++ | |
IgG2c | ++ | ++ | +++ | |
IgG3 | + | ++ | ? | |
Mouse | IgG | ++ | ++ | +++ |
IgG1 | + | ++++ | +++ | |
IgG2a | ++++ | ++++ | +++ | |
IgG2b | +++ | +++ | +++ | |
IgG3 | ++ | +++ | +++ | |
IgM | – | – | +++ | |
Chicken | IgY | – | – | – |
Monkey | IgG | ++++ | ++++ | ? |
Hamster | + | ++ | +++ | |
Koala | – | + | ? | |
Llama | – | + | ? |
Strong binding ++, medium interaction +, weak or no interaction ï¼.
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