Biotinylated Recombinant Protein A/G

Biotinylated Recombinant Protein A/G

Catalogue #: AX-AP1101B

Size: 0.5 mg

Description: Approximately 50.5 kDa. Recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C2C3, which corresponds to Protein A and G domains that are included in the recombinant sequence. Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. Protein G portion is from Streptococcus segments C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Specificity: Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG. The extended Fc-binding properties of Protein A/G make it a popular tool in the investigation and purification of immunoglobulins. Protein A/G binds to all human IgG subclasses, IgA, IgE, IgM and to a lesser extent IgD; however, it does not bind mouse IgA, IgM or murine serum albumin.

Formulation: Lyophilized from a 0.2μm filtered solution of 20mM PBS, pH 7.0, containing 2% mannitol.

Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C.

Storage: This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C.Avoid repeated freeze/thaw cycles.

Usage: For research, laboratory or further evaluation purposes.

IgG Binging to Protein A, G and L:

Strong binding ＋＋, medium interaction ＋, weak or no interaction －.