7-aminoactinomycin D (7-AAD) is a fluorescent intercalator that undergoes a spectral shift upon association with DNA. 7-AAD/DNA complexes can be excited by the 488 nm laser and has an emission maxima of 647 nm, making this nucleic acid stain useful for multicolor fluorescence microscopy and flow cytometry. 7-AAD appears to be generally excluded from live cells, but can be used with cells that have been fixed and permeabilized. 7-AAD has been used for cell cycle analysis by flow cytometry. 7-AAD also binds selectively to GC regions of DNA yielding a distinct banding pattern in polytene chromosomes and chromatin for use in chromosome banding studies..
Molecular formula: C62H87N13O16 ; Molecular weight: 1270.45 ; CAS name/number: Actinomycin D, 7-amino- 7240-37-1
|For use with(equipment)||Flow cytometer;Fluorescence Mciroscope|
|Sub-Cellular Localization||Nucleic Acids, Nucleus|
|Flow Cytometer Laser Lines:||488|
|Label or Dye:||7-AAD|
|Form:||Solid and li|
1mg ; 2mL
Store in freezer (-5 to -30°C) and protect from light.Shipping in room temperature
Celltechgen provides high-quality reagents and materials for research use only. 7-AAD is a potential carcinogen. It is recommended that the user wear gloves, protective clothing, and eye/face protection in order to avoid contact with skin and eyes. For proper handling of potentially hazardous chemicals, please request the Safety Data Sheet (SDS) provided for the product.
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